Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (C-Term) antibody

Details for Product No. ABIN374246
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Antigen
Synonyms GB11574, rse1, sap130, sf3b130, staf130, sf3b3, 1810061H24Rik, 5730409A01Rik, AA409318, D8Ertd633e, RSE1, SAP130, mKIAA0017, ik:tdsubc_2b2, wu:fb81f05, xx:tdsubc_2b2, zgc:55440, SF3b130, STAF130
Epitope
C-Term
(13), (11), (8), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(44), (16), (6), (2), (1), (1), (1)
Host
Goat
(38), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(42), (26), (7), (5), (4), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374246
357.50 $
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Immunogen Peptide from C Terminus of the protein sequence according to NP_036558.3 Genename: SF3B3
Sequence C-VSKKLEDIRT RYAF
Specificity This antibody detects SAP130 at C-term. Species Reactivity: Tested: Human Expected from sequence similarity: Mouse, Rat, Dog, Cow
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name SF3B3 / SAP130
Background Alternate names: KIAA0017, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP 130, SAP-130, SF3b130, STAF130, Spliceosome-associated protein 130, Splicing factor 3B subunit 3
Gene ID 23450
NCBI Accession NP_036558
UniProt Q15393
Application Notes Peptide ELISA: Limit dilution 1: 2000. Western blot: 0.1-0.3 µg/ml. Approx 150kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 136kDa according to NP_036558.3). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months
Supplier Images
anti-Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (C-Term) antibody anti-Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) (C-Term) antibody
Background publications Das, Xia, Palandjian et al.: "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6796-802, 2000 (PubMed).

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