RAB11A antibody (C-Term)

Catalog No. ABIN374252

The Goat Polyclonal anti-RAB11A antibody is suitable to detect RAB11A in samples from Human, Mouse, Rat and Cow. It has been validated for WB.

Quick Overview for RAB11A antibody (C-Term) (ABIN374252)

Target: RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This RAB11A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAB11A Antibody

Binding Specificity: C-Term

Sequence: TENKPKVQCC QNI

Specificity: This antibody detetcs RAB11A at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_004654.1 Genename: RAB11A

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Mouse Heart lysates (CalculatedMW of 24.6 kDa according to NP_004654.1). In transfected HEK293 transiently expressingRAB11A a band of approx.26 kDa is observed. This band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAB11A

Target: RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))

Alternative Name: RAB11A / RAB11

Background: Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.Synonyms: RAB-11, Ras-related protein Rab-11A, YL8

Gene ID: 8766

NCBI Accession: NP_004654

UniProt: P62491

Pathways: Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity