Dynactin 1 antibody (C-Term)

Catalog No. ABIN374269

Product Details anti-Dynactin 1 Antibody

Target: Dynactin 1 (DCTN1)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-QEQLHQLHSR LIS

Specificity: This antibody is expected to recognise all reported isoforms of Dynactin subunit 1 (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1).

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1. Genename: DCTN1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 128000. Western Blot: 0.5-2 μg/mL. pprox 150 kDa band observed in cell lline A549 and HumanTestis lysates (calculated MW of 140 kDa according to NP_004073.2). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Cerebellum showspixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testisshows cytoplasm staining in cells of the seminiferous tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: Dynactin Subunit 1 (DCTN1 Products)

Synonyms: DAP-150 antibody, DP-150 antibody, P135 antibody, Chip150 antibody, DCTN1 antibody, DKFZp469C1813 antibody, AL022633 antibody, Glued antibody, p150 antibody, p150 antibody, CG9206 antibody, DYNA_DROME antibody, Dmel\\CG9206 antibody, FCP-D antibody, Glu antibody, P150 antibody, P150[Glued] antibody, p150/Glued antibody, p150/glued antibody, p150[Glued] antibody, p150[glued] antibody, t1 antibody, Dynactin-1 antibody, glued antibody, dynactin subunit 1 antibody, dynactin 1 antibody, Dynactin 1, p150 subunit antibody, dynactin subunit 1 L homeolog antibody, DCTN1 antibody, Dctn1 antibody, DCTN1-p150 antibody, dctn1.L antibody

Background: The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.Synonyms: 150 kDa dynein-associated polypeptide, DAP-150, DCTN1, DP-150, p135, p150-glued

Gene ID: 1639

NCBI Accession: NP_004073

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling