PUF60 antibody (C-Term)
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- Target See all PUF60 Antibodies
- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Dog, Cow
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PUF60 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-YDQERFDNSD LSA
- Specificity
- This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1, NP_055096.2, NP_001129505.1)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Rat, Dog, Cow.
Species reactivity (tested):Human. - Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from C Terminus of the protein sequence according to NP_510965.1, NP_055096.2, NP_001129505.1 Genename: PUF60
- Top Product
- Discover our top product PUF60 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysate of human epidermoidcarcinoma A431 (calculated MW of 59.0 kDa (isoform A) according to NP_510965.1 and58.0 kDa (isoform B) according to NP_055096.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
- Alternative Name
- PUF60 / SIAHBP1 (PUF60 Products)
- Synonyms
- FIR antibody, RoBPI antibody, SIAHBP1 antibody, fc21a05 antibody, wu:fc21a05 antibody, wu:fi43a08 antibody, 2410104I19Rik antibody, 2810454F19Rik antibody, Siahbp1 antibody, fe37c05 antibody, repressor antibody, si:ch211-12p8.2 antibody, si:zc12p8.2 antibody, wu:fb33e11 antibody, wu:fe37c05 antibody, zgc:86806 antibody, poly(U) binding splicing factor 60 antibody, poly-U binding splicing factor a antibody, poly-U binding splicing factor 60 antibody, poly-U binding splicing factor b antibody, PUF60 antibody, puf60a antibody, Puf60 antibody, puf60b antibody
- Background
- Synonyms: FBP-interacting repressor, FIR, Poly(U)-binding-splicing factor PUF60, ROBPI, Ro-binding protein 1, Siah-binding protein 1
- Gene ID
- 22827
- NCBI Accession
- NP_510965
- UniProt
- Q9UHX1
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