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Ube2t antibody (Ubiquitin-Conjugating Enzyme E2T (Putative)) (C-Term)

Details for Product anti-Ube2t Antibody No. ABIN374292, Supplier: Log in to see
Antigen
  • UBE2T
  • wu:fj86h11
  • zgc:153687
  • HR6B
  • PIG50
  • 2700084L22Rik
  • C80607
  • RGD1310816
  • hspc150
  • ubiquitin-conjugating enzyme E2T (putative)
  • Ubiquitin-conjugating enzyme E2 T
  • ubiquitin-conjugating enzyme E2T
  • similar to Ubiquitin-conjugating enzyme E2 T (Ubiquitin-protein ligase T) (Ubiquitin carrier protein T)
  • ubiquitin-conjugating enzyme E2B
  • UBE2T
  • ube2t
  • LOC100356060
  • LOC100357338
  • Ube2b
  • Ube2t
Alternatives
anti-Human Ube2t antibody for ELISA
Epitope
C-Term
24
14
9
6
6
5
4
3
1
1
1
Reactivity
Human
78
13
7
1
1
1
Host
Goat
45
17
16
Clonality
Polyclonal
Conjugate
This Ube2t antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
74
48
9
9
2
2
2
1
Options
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Immunogen Peptide from C Terminus of the protein sequence according to NP_054895.1. Genename: UBE2T
Sequence C-QLVGIEKKFH PDV
Specificity This antibody detects UBE2T at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name UBE2T (Ube2t Antibody Abstract)
Background Synonyms: HSPC150, Ubiquitin carrier protein T, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T
Gene ID 29089
NCBI Accession NP_054895
UniProt Q9NPD8
Pathways Chromatin Binding
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx.22 kDa band observed in lysates of cell lines K562,HEK293 and A549 (calculated MW of 22.2 kDa according to NP_054895.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-Ube2t antibody (Ubiquitin-Conjugating Enzyme E2T (Putative)) (C-Term) (ABIN374292) anti-Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody
Background publications Machida, Machida, Chen, Gurtan, Kupfer, DAndrea, Dutta: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).