CLPP antibody (C-Term)

Catalog No. ABIN374308

Product Details anti-CLPP Antibody

Target: CLPP (ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli) (CLPP))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLPP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-EAAPAAEPVP AST

Specificity: This antibody detects CLPP at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006003.1 Genename: CLPP

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: 1-3 μg/mL. Approx 27-30 kDa band observed in human heart and skeletalmuscle lysates (predicted MW of 30 kDa according to NP_006003). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Liver shows patternedstaining of hepatocyte cytoplasm.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CLPP

Target: CLPP (ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli) (CLPP))

Alternative Name: CLPP (CLPP Products)

Synonyms: CLPP antibody, MGC130667 antibody, AU019820 antibody, D17Wsu160e antibody, PRLTS3 antibody, caseinolytic mitochondrial matrix peptidase proteolytic subunit antibody, caseinolytic mitochondrial matrix peptidase proteolytic subunit L homeolog antibody, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog antibody, CLPP antibody, clpp.L antibody, clpp antibody, Clpp antibody

Background: CLPP belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.Synonyms: Endopeptidase Clp, Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

Gene ID: 8192

NCBI Accession: NP_006003

UniProt: Q16740