Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody

Details for Product No. ABIN374314
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Antigen
Synonyms CARD11, LOC100232755, BENTA, BIMP3, CARMA1, PPBL, 0610008L17Rik, 2410011D02Rik
Epitope
C-Term
(13), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine)
(59), (36), (13), (13), (13), (12)
Host
Goat
(55), (6), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(50), (26), (19), (15), (10), (4), (3), (3), (2)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374314
357.50 $
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Immunogen Peptide from the C Terminus of the protein sequence according to NP_115791.3 Genename: CARD11
Sequence C-QRKTIWVDED QL
Specificity This antibody detects CARD11 / CARMA1 at C-term.
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name CARD11 / CARMA1
Background CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
Alternate names: CARD-containing MAGUK protein 3, Caspase recruitment domain-containing protein 11
Gene ID 84433
NCBI Accession NP_115791
UniProt Q9BXL7
Research Area Apoptosis/Necrosis
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western Blot: 1-3 µg/ml. Approx 150+65kDa bands observed in lysates of cell line Jurkat(calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed inHEK293 transiently expressing CARD11. These bands are not observed in thenon-transfected HEK293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months
Supplier Images
anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody
anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (2) anti-Caspase Recruitment Domain Family, Member 11 (CARD11) (C-Term) antibody (Image 2)
Background publications Bertin, Wang, Guo et al.: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001 (PubMed).

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