IFIH1
Reactivity: Human
WB
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Peptide ELISA: Limit dilution 1: 32000. Immunohistochemistry: 10 μg/mL. In paraffin embedded Human Pancreas, shows strongcytoplasmic staining of some cells in the islets of Langerhans. In Western blot no signal obtained yet but low background observed in Human Placentalysate at up to 1 μg/mL.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
IFIH1
(Interferon Induced with Helicase C Domain 1 (IFIH1))
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents, treatment with either agent alone only achieves reversible differentiation. It is believed that MDA-5 may function to promote message degradation by specific RNases. It also seems to possess growth inhibition properties. The interaction of MDA5 with double stranded RNA can lead to induction of IFN required for the the innate immune response against viruses.Synonyms: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116, RNA helicase-DEAD box protein 116