FOXA1 antibody (C-Term)

Catalog No. ABIN374334

This anti-FOXA1 antibody is a Goat Polyclonal antibody detecting FOXA1 in WB, IHC (p), ChIP and EIA. Suitable for Human, Rat, Mouse, Cow and Pig.

Quick Overview for FOXA1 antibody (C-Term) (ABIN374334)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Product Details anti-FOXA1 Antibody

Binding Specificity: C-Term

Sequence: C-GVYSRPVLNT S

Specificity: This antibody detects FOXA1.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Pig, Cow.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_004487.2 Genename: FOXA1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1/16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in Human lysates of cell lineHepG2 (calculated MW of 49.0 kDa according to NP_004487.2). An additional band ofunknown identity was also consistently observed at 30 kDa. This band was successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Anonymous customers used this product successfully in ChIP with MCF7.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Alternative Name: FOXA1 / TCF3A

Background: FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.Synonyms: Forkhead box protein A1, HNF-3-alpha, HNF-3A, HNF3A, Hepatocyte nuclear factor 3-alpha, TCF-3A, Transcription factor 3A

Molecular Weight: 49.1 kDa (NP_004487.2)

Gene ID: 3169

NCBI Accession: NP_004487

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis