You are viewing an incomplete version of our website. Please click to reload the website as full version.

SMARCE1 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1) (C-Term)

Details for Product anti-SMARCE1 Antibody No. ABIN374349, Supplier: Log in to see
Antigen
  • BAF57
  • XBaf57
  • MGC52696
  • MGC69429
  • MGC81726
  • SMARCE1
  • DKFZp459O2235
  • fb54d11
  • wu:fb33d02
  • wu:fb54d11
  • Baf57
  • 2810417B20Rik
  • 5830412H02Rik
  • 9030408N19Rik
  • Baf27
  • RGD1304726
Epitope
C-Term
39
15
4
4
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
79
14
10
4
2
2
2
1
1
1
1
Host
Goat
49
18
12
Clonality
Polyclonal
Conjugate
This SMARCE1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
67
41
39
12
11
11
5
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Peptide from C Terminus of the protein sequence according to NP_003070.3 Genename: SMARCE1
Sequence C-PPTDPIPEDE KKE
Specificity This antibody detects SMARCE1 / BAF57 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):MouseSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name SMARCE1 / BAF57 (SMARCE1 Antibody Abstract)
Background Synonyms: BRG1-associated factor 57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Gene ID 6605
NCBI Accession NP_003070
UniProt Q969G3
Research Area Apoptosis/Necrosis
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEPG2,HeLa and Jurkat (calculated MW of 46.0 kDa according to NP_003070.3). A 40 kDa band wasobserved in transfected HEK293 transiently expressing BAF57. This band is not observed inthe non-transfected HEK293. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EABIN374349 (mocktransfection in first lane), tested by Origene. B) ABIN374349 staining (0.2 μg/mL) of Jurkat lysate (RIPA buffer, 35 μg total protein perlane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-SMARCE1 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1) (C-Term) (ABIN374349) anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1) (C-Term) antibody
 image for anti-SMARCE1 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1) (C-Term) (ABIN374349) anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1) (C-Term) antibody (Image 2)
Background publications Wang, Chi, Xue et al.: "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 492-8, 1998 (PubMed).

Did you look for something else?