Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Sequence
DGIVPDIAVG TKR-C
Specificity
This antibody is expected to recognise all 4 reported isoforms of Human PTBP1 (PTB) (represented by NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Bovine. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide from N-Terminus of the protein sequence according to NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1. Genename: PTBP1
Peptide ELISA: Limit dilution 1: 8000. Western blot: 1 μg/mL. Approx 60-65 kDa band observed in Jurkat lysate (predicted MW of64-, 63- and 61- kDa according to NP_002810, NP_114367 and NP_114368). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.