FHL2 antibody (C-Term)

Catalog No. ABIN374413

Product Details anti-FHL2 Antibody

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RDDILCPDCG KDI

Specificity: This antibody detects FHL2 / SLIM3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_001441.4, NP_963849.1, NP_963851.2, NP_001034581.1. Genename: FHL2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 30 kDa band observed in Human Heart lysates (calculatedMW of 32.0 kDa according to NP_001441.4).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for FHL2

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Alternative Name: FHL2 / SLIM3 (FHL2 Products)

Synonyms: AAG11 antibody, DRAL antibody, FHL-2 antibody, SLIM-3 antibody, SLIM3 antibody, C76204 antibody, FHL2 antibody, zgc:92428 antibody, zgc:103466 antibody, four and a half LIM domains 2 antibody, four and a half LIM domains 2a antibody, four and a half LIM domains 2b antibody, FHL2 antibody, Fhl2 antibody, fhl2 antibody, fhl2a antibody, fhl2b antibody

Background: FHL2 is a LIM protein, all of which contain a highly conserved double zinc finger motif called the LIM domain. It is thought to be a co-activator of the androgen receptor and a transcription coactivator. FHL2 might enforce beta-catenin transactivation activity in cancer cells and may be downregulated during transformation of a variety of cell types.Synonyms: DRAL, FHL-2, Four and a half LIM domains protein 2, LIM domain protein DRAL, SLIM 3, Skeletal muscle LIM-protein 3

Gene ID: 2274

NCBI Accession: NP_001441

UniProt: Q14192

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha