MPG antibody (C-Term)

Catalog No. ABIN374423

The Goat Polyclonal anti-MPG antibody is suitable to detect MPG in samples from Human. It has been validated for WB.

Quick Overview for MPG antibody (C-Term) (ABIN374423)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MPG antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MPG Antibody

Binding Specificity: C-Term

Sequence: C-SVVDRVAEQD TQA

Specificity: This antibody detects MPG / ANPG at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002425.2, NP_001015052.1, NP_001015054.1. Genename: MPG

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 28-30 kDa band observed in lysates of cell line K562(calculated MW of 30.0 kDa according to NP_001015054.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Alternative Name: MPG / ANPG

Background: MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.Synonyms: 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG, DNA-3-methyladenine glycosylase, MID1, N-methylpurine-DNA glycosylase

Gene ID: 4350

NCBI Accession: NP_002425

UniProt: P29372

Pathways: DNA Damage Repair