DEF6 antibody (N-Term)

Catalog No. ABIN374432

Product Details anti-DEF6 Antibody

Target: DEF6 (Differentially Expressed in FDCP 6 Homolog (DEF6))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This DEF6 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ALRKELLKSI WYA-C

Specificity: This antibody recognizes IBP/DEF6.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Cow, Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_071330.3

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/2,000. Western Blot: 1-3 μg/mL. Approx.70 kDa band observed in Human Thymus lysates(calculated MW of 73.0 kDa according to NP_071330.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DEF6

Target: DEF6 (Differentially Expressed in FDCP 6 Homolog (DEF6))

Alternative Name: DEF6 (DEF6 Products)

Synonyms: DEF6 antibody, Def6 antibody, zgc:63721 antibody, ibp antibody, slat antibody, swap70l antibody, MGC68775 antibody, 2410003F05Rik antibody, 6430538D02Rik antibody, AV094905 antibody, Ibp antibody, Slat antibody, Slat2 antibody, Slat6 antibody, IBP antibody, SLAT antibody, SWAP70L antibody, DEF6, guanine nucleotide exchange factor antibody, differentially expressed in FDCP 6a homolog (mouse) antibody, DEF6, guanine nucleotide exchange factor L homeolog antibody, differentially expressed in FDCP 6 antibody, DEF6 guanine nucleotide exchange factor antibody, DEF6 antibody, def6a antibody, def6.L antibody, def6 antibody, Def6 antibody

Background: DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC and CDC42 that is highly expressed in B and T cells. It is a novel PH-DH-like domain protein, and is an upstream activator of the Rho GTPases Rac1, Cdc42, and RhoA.Synonyms: DEF-6, Differentially expressed in FDCP 6 homolog, IBP, IRF4-binding protein, SLAT, SWAP70L

Gene ID: 50619

NCBI Accession: NP_071330

UniProt: Q9H4E7