MCL-1 antibody (C-Term, Isoform 1)

Catalog No. ABIN374436

This anti-MCL-1 antibody is a Goat Polyclonal antibody detecting MCL-1 in WB. Suitable for Human, Mouse, Rat, Dog and Cat.

Quick Overview for MCL-1 antibody (C-Term, Isoform 1) (ABIN374436)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human, Mouse, Rat, Dog, Cat

Host: Goat

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCL-1 Antibody

Binding Specificity: C-Term, Isoform 1

Sequence: C-AGVGAGLAYL IR

Specificity: This antibody is expected to recognise isoform 1 (NP_068779.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Feline (Cat).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_068779.1

Application Details

Application Notes: Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of Human Lung and cellline K562 (calculated MW of 37.5 kDa according to NP_068779.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1

Background: Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004

Gene ID: 4170

NCBI Accession: NP_068779

UniProt: Q07820

Pathways: MAPK Signaling