LAT antibody (C-Term)

Catalog No. ABIN374446

This Goat Polyclonal antibody specifically detects LAT in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LAT antibody (C-Term) (ABIN374446)

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LAT Antibody

Binding Specificity: C-Term

Sequence: C-GAPDYENLQE LN

Specificity: This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1

Application Details

Application Notes: Peptide ELISA: 1/2,000. Western Blot: 1-3 μg/mL. Detects a band of Approx 35 kDa in Human PBMC lysates(Predicted Molecular Weight: 35 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT

Background: Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36

Molecular Weight: 27.9kDa (NP_055202.1).

Gene ID: 27040

NCBI Accession: NP_001014987

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway