This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
Cross-Reactivity (Details)
Species reactivity (expected):Rat, Canine, Porcine and Bovine. Species reactivity (tested):Human, Mouse.
Purification
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogen
Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.1, NP_001153171.1. Genename: FHL1
FHL1
Reactivity: Human
WB, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.05-0.3 μg/mL. Detects a band of Approx 30 kDa in Human Muscle lysates(calculated MW of 31.0 kDa according to NP_001440). Immunohistochemistry: 2-2 μg/mL. In paraffin embedded Human Testis shows fibrousstaining in the cytoplasm of developing sperm cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
fhl1 antibody, MGC89165 antibody, FHL1 antibody, DKFZp468O1927 antibody, fhl antibody, fhla antibody, zgc:92025 antibody, FHL-1 antibody, FHL1A antibody, FHL1B antibody, FLH1A antibody, KYOT antibody, SLIM antibody, SLIM-1 antibody, SLIM1 antibody, SLIMMER antibody, XMPMA antibody, KyoT antibody, Fhl1 antibody, four and a half LIM domains 1 antibody, four and a half LIM domains 1a antibody, Four and a half LIM domains protein 1 antibody, four and a half LIM domains 1 S homeolog antibody, fhl1 antibody, FHL1 antibody, fhl1a antibody, fhl1.S antibody, Fhl1 antibody
Background
FHL1 (SLIM1) is a LIM domain protein transcript that is preferentially expressed in postnatal skeletal muscle but little is known about its function in this tissue. LIM proteins, (named for 'LIN11, ISL1 and MEC3') are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.Synonyms: FHL-1, FHL1, Four and a half LIM domains protein 1, SLIM, SLIM-1, SLIM1, Skeletal Muscle LIM-protein 1