POMC antibody (C-Term)

Catalog No. ABIN374549

Product Details anti-POMC Antibody

Target: POMC (Proopiomelanocortin (POMC))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This POMC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-NAIIKNAYKK GE

Specificity: In Western blot, this antibody appears to react with the full length precursor protein only.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human and Mouse.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic Peptide corresponding to C-Terminal of Human POMC (according to NP_000930.1, NP_001030333.1) Genename: POMC

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/32,000. Western Blot: Use at 0.01-0.03 μg/mL. Detects a band of approx. 26 kDa band in lysates of Mouse pituitary cell line NIH3T3. Immunohistochemistry: Use at 2-4 μg/mL. In Paraffin Embedded Human Adrenal Gland shows strong cytoplasm staining in selectcells in the medulla.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC (POMC Products)

Synonyms: MSH antibody, ACTH antibody, pomc antibody, SI:bZ36D5.1 antibody, SI:bZ36D5.4 antibody, si:bz36d5.2 antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, NPP antibody, POC antibody, Pomc2 antibody, proopiomelanocortin a antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, pomca antibody, Pomc antibody, POMC antibody

Background: POMC is a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used, adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. Mutations in POMC gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.Synonyms: Corticotropin-Lipotropin, Pro-opiomelanocortin

Molecular Weight: 29.4kDa (Human NP_000930.1) and 26.7kDa (Mouse NP_032921.1).

Gene ID: 5443, 9606

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour