ENPEP antibody (Internal Region)

Catalog No. ABIN374574

The Goat Polyclonal anti-ENPEP antibody (ABIN374574) specifically detects ENPEP in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat, Pig and Dog samples.

Quick Overview for ENPEP antibody (Internal Region) (ABIN374574)

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPEP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ENPEP Antibody

Binding Specificity: Internal Region

Sequence: C-EQYQKTSLAQ EKEK

Specificity: This antibody reacts to CD249. Approx 170 kDa band observed in Human Brain, Kidney and Testis lysates (calculated MW of 109 kDa according to NP_001968.2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence from the internal region of the protein

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: 3 - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: CD249 / Glutamyl Aminopeptidase

Background: BP1, also known as Ly51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide linked chains of 140 kDa. Originally identified on the surface of pre B cell lymphomas the cDNA encoding BP1 has been cloned and found to represent aminopeptidase A. It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity. Subsets of normal bone marrow pre B and B cells express low levels of BP1, which is rapidly upregulated on pre B cells in the presence of IL7.Synonyms: Aminopeptidase A, Differentiation antigen gp160, EAP, ENPEP

Gene ID: 2028, 9606

UniProt: Q07075

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones