This antibody is expected to recognise Cyclophilin A. This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide with sequence from the internal region of the protein sequence according to NP_066953.1. Genename: PPIA
PPIA
Reactivity: Human, Mouse, Rat
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 μg/mL. Approx 18 kDa band observed in Human Lymph Node lysates(calculated MW 18.8 kDa according to NP_066953.1).
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
PPIA
(Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
Cyp1 antibody, PPIA-2 antibody, fa93g09 antibody, ppia antibody, wu:fa93g09 antibody, wu:fb05e11 antibody, wu:fb13h02 antibody, wu:fb15a05 antibody, CYPA antibody, CypA antibody, Cypa antibody, CYPH antibody, CYCA antibody, CyP-A antibody, MGC75715 antibody, 2700098C05 antibody, Cphn antibody, CyP-18 antibody, MT-ND1 antibody, MTND1 antibody, NADH1 antibody, ND1 antibody, cypA antibody, PPIase A antibody, ppial antibody, wu:fj18g05 antibody, zgc:73102 antibody, zgc:86688 antibody, peptidylprolyl isomerase A antibody, peptidylprolyl isomerase Aa (cyclophilin A) antibody, peptidylprolyl isomerase A (cyclophilin A) L homeolog antibody, cyclophilin A antibody, cyclophilin a antibody, peptidylprolyl isomerase A (cyclophilin A) antibody, peptidylprolyl isomerase Ab (cyclophilin A) antibody, PPIA antibody, ppiaa antibody, ppia.L antibody, Cypa antibody, CNB01230 antibody, CNB01290 antibody, Tc00.1047053506925.300 antibody, Tb11.03.0250 antibody, CYPA antibody, Ppia antibody, ppia antibody, ppiab antibody
Background
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.Synonyms: CYPA, Cyclophilin-A, Cyclosporin A-binding protein, EC=5.2.1.8, PPIA, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Rotamase A