Melanoma gp100 antibody (C-Term)

Catalog No. ABIN374704

This Goat Polyclonal antibody specifically detects Melanoma gp100 in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Melanoma gp100 antibody (C-Term) (ABIN374704)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes Silver homologue / Pmel17.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Melanoma Gp100

Background: Melanoma gp100, a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.Synonyms: D12S53E, ME20-M, Melanocyte lineage-specific antigen GP100, Melanocyte protein Pmel 17, Melanoma-associated ME20 antigen, PMEL17, SILV, Silver locus protein homolog

Molecular Weight: 70.3kDa (NP_008859.1).

Gene ID: 6490

NCBI Accession: NP_001186982

UniProt: P40967