Peptide ELISA: 1/128000. Western Blot: 0.1 - 0.3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
FE65 (APBB1)
(Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))
FE65 antibody, MGC:9072 antibody, RIR antibody, apbb1 antibody, MGC80654 antibody, Fe65 antibody, Rir antibody, amyloid beta precursor protein binding family B member 1 antibody, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) L homeolog antibody, amyloid beta (A4) precursor protein-binding, family B, member 1 antibody, APBB1 antibody, apbb1.L antibody, Apbb1 antibody
Background
Amyloid precursor protein (APP), which plays a central role in the pathogenesis of Alzheimer's disease (AD), is a cell-surface protein that is cleaved by gamma-secretase at the transmembrane region into an extracellular amyloid-beta peptide (Ab) and an intracellular tail fragment. This cytoplasmic tail of APP forms a multimeric complex with the nuclear adaptor protein FE65 and the histone acetyltransferase Tip60.1 FE65 is an adaptor protein that bridges APP to certain molecular pathways. FE65 is also highly expressed in neurons and possesses the characteristics of a transcription factor. The interaction between APP and FE65 increases the translocation of APP to the cell surface and the subsequent secretion of Ab. FE65 is thus important in the regulation and trafficking of APP.Synonyms: Amyloid beta A4 precursor protein-binding family B member 1, RIR