DIO2 antibody (Internal Region)

Catalog No. ABIN374755

This Goat Polyclonal antibody specifically detects DIO2 in IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for DIO2 antibody (Internal Region) (ABIN374755)

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This DIO2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DIO2 Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognise both reported isoform a and isoform b of DIO2.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat and Porcine.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography

Immunogen: Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: Detection limit: 1/128000. Western Blot: Preliminary experiments gave an approx 65-70 kDa band in Human Heart andThyroid lysates after 0.05 μg/mL DIO2 antibody staining. Immunohistochemistry: 2-3 μg/mL. Cryo-sectioned Mouse Brain (Hippocampus) showsstaining in the granular layer of the dentate gyrus.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Alternative Name: DIO2

Background: DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms.Synonyms: DIOII, ITDI2, TXDI2, Type II iodothyronine deiodinase, Type-II 5'-deiodinase

Gene ID: 1734

NCBI Accession: NP_000784

UniProt: Q92813

Pathways: Hormone Transport, Hormone Activity