CCKAR antibody (Internal Region)
-
- Target See all CCKAR Antibodies
- CCKAR (Cholecystokinin A Receptor (CCKAR))
-
Binding Specificity
- Internal Region
-
Reactivity
- Rat, Mouse, Rabbit
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This CCKAR antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- C-KFDASQKKSA KEKR
- Specificity
- This antibody detects CCK-A Receptor.
- Cross-Reactivity (Details)
- Species reactivity (expected):Mouse, RabbitSpecies reactivity (tested):Rat.
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from the internal region of the protein sequence according to NP_033957.1. Genename: Cckar
- Top Product
- Discover our top product CCKAR Primary Antibody
-
-
- Application Notes
-
Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.1-0.3 μg/mL. Approx.75 kDa band observed in purified membranes of Ratinsulinoma cell line RIN14B (calculated MW of 48.5 kDa according to NP_033957.1). Datakindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by InternationalJournal of Endocrinology (in press). The observed molecular weight also corresponds toearlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem.2003 Nov,51(11): 1501-13, PMID: 14566022).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide, with 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- CCKAR (Cholecystokinin A Receptor (CCKAR))
- Alternative Name
- CCK-A Receptor (CCKAR Products)
- Synonyms
- CCK-XL antibody, cck-a antibody, cck1-r antibody, cckar-A antibody, cckbr antibody, cckra antibody, CCKAR antibody, CCK-A antibody, CCK1-R antibody, CCKRA antibody, AW106902 antibody, Cck-ar antibody, CCK-AR antibody, cholecystokinin A receptor antibody, cholecystokinin A receptor L homeolog antibody, CCKAR antibody, cckar.L antibody, Cckar antibody
- Background
- The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.Synonyms: CCKA Receptor, CCKAR, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor
- Gene ID
- 886, 9606
- UniProt
- P32238
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour
-