CRHR1 antibody (Internal Region)

Catalog No. ABIN374874

Product Details anti-CRHR1 Antibody

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Binding Specificity: AA 107-117, Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CRHR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: C-NEEKKSKVHY H

Specificity: This antibody detects CRHR1.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_004373.2 Genename: CRHR1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 60-70 kDa band observed in human brain (cerebellum)and human colon lysates (calculated MW of 47.0 kDa according to NP_004373.2). Theobserved molecular weight corresponds to earlier findings in literature with differentantibodies (Xu et al, Mol Pharmacol.2001 Mar,59(3): 485-92, PMID: 11179443) . Immunofluorescence: 2 μg/mL when incubated at 4 °C for 40h (mouse brain).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Alternative Name: CRHR1 (CRHR1 Products)

Synonyms: CRF-R antibody, CRF-R-1 antibody, CRF-R1 antibody, CRF1 antibody, CRFR-1 antibody, CRFR1 antibody, CRH-R-1 antibody, CRH-R1 antibody, CRH-R1h antibody, CRHR antibody, CRHR1L antibody, CRHR1f antibody, Crhr antibody, crhr1 antibody, crhr1-A antibody, CRHR1 antibody, CRHR1-IT1-CRHR1 antibody, MGC57346-CRHR1 antibody, corticotropin releasing hormone receptor 1 antibody, corticotropin releasing hormone receptor 1, gene 2 S homeolog antibody, corticotropin-releasing factor receptor 1 antibody, LINC02210-CRHR1 readthrough antibody, CRHR1 antibody, Crhr1 antibody, crhr1.2.S antibody, LOC610380 antibody, LOC100065598 antibody, LINC02210-CRHR1 antibody, crhr1 antibody

Background: CRF1 is a receptor for corticotropin releasing factor. It shows high affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.Synonyms: CRF-R, CRF1, CRFR, CRFR1, CRH-R 1, CRHR, CRHR-1, Corticotropin-releasing factor receptor 1, Corticotropin-releasing hormone receptor 1

Gene ID: 1394

NCBI Accession: NP_001138618

UniProt: P34998

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity