CRHR1 antibody (N-Term)

Catalog No. ABIN213513

The Rabbit Polyclonal anti-CRHR1 antibody (ABIN213513) specifically detects CRHR1 in IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Dog, Horse, Monkey and Hamster samples.

Quick Overview for CRHR1 antibody (N-Term) (ABIN213513)

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRHR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CRHR1 Antibody

Binding Specificity: N-Term

Specificity: Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CRHR2 (73 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Panda, Horse (100%) Bat, Bovine, Pig, Opossum (93%) Elephant (87%) Sheep, Lizard (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-terminal extracellular domain of human CRF1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Panda, Horse (100%), Bat, Bovine, Pig, Opossum (93%), Elephant (87%), Sheep, Lizard (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (15 - 20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Alternative Name: CRFR1 / CRHR1

Background: Name/Gene ID: CRHR1
Subfamily: Corticotropin releasing factor
Family: GPCR

Synonyms: CRHR1, CRF-R-1, CRFR, CRHR1f, Crf receptor 1, CRH-R1, CRF-R, CRF1, CRFR-1, Crf receptor type 1, Crf receptor type 1, Crf type 1, CRF-R1, Crf1 receptor, CRFR1, CRH-R-1, CRH-R1h, CRHR, CRHR1L

Gene ID: 1394

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity