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MARCH6 antibody (Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase) (Internal Region)

Details for Product anti-MARCH6 Antibody No. ABIN374941, Supplier: Log in to see
Antigen
  • RGD1310927
  • RGD1565757
  • im:7161807
  • wu:fa98a02
  • si:dkey-40c17.2
  • Mar-06
  • 3830408G03
  • F830029L24Rik
  • mKIAA0597
  • DOA10
  • MARCH-VI
  • RNF176
  • TEB4
Epitope
Internal Region, AA 701-712
12
11
7
7
4
3
1
1
Reactivity
Human, Mouse (Murine)
50
19
14
1
1
1
1
1
1
1
Host
Goat
31
14
6
Clonality
Polyclonal
Conjugate
This MARCH6 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
34
24
11
6
2
1
Supplier
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Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_005876.2 Genename: MARCH6
Sequence C-QGRRVIFQKV KE
Specificity This antibody recognizes TEB4 / MARCH-VI (701-712).
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name MARCH6 / RNF176 (MARCH6 Antibody Abstract)
Background MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.Synonyms: Doa10 homolog, E3 ubiquitin-protein ligase MARCH6, KIAA0597, MARCH-VI, Membrane-associated RING finger protein 6, Membrane-associated RING-CH protein VI, Protein TEB-4, RING finger protein 176, TEB-4, TEB4
Gene ID 10299, 9606
UniProt O60337
Application Notes Peptide ELISA: 1/16000. Western Blot: 0.2 - 0.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0,5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-MARCH6 antibody (Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase) (Internal Region) (ABIN374941) anti-Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6) (AA 701-712), (Internal Region) antibody
Background publications Kreft, Wang, Hochstrasser: "Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 4646-53, 2006 (PubMed).

Buckley, Bonner, Brann: "Localization of a family of muscarinic receptor mRNAs in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 12, pp. 4646-52, 1989 (PubMed).

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