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GTPase NRas antibody (NRAS) (Internal Region)

Details for Product anti-NRAS Antibody No. ABIN375000, Supplier: Login to see
Antigen
  • NRAS
  • AV095280
  • N-ras
  • ALPS4
  • NRAS1
  • NS6
  • N-RAS
  • fj99a10
  • wu:fj99a10
  • zgc:100761
  • zras-b1
  • H-RAS
  • HRAS
Alternatives
anti-Human GTPase NRas antibody for Western Blotting
Epitope
Internal Region
10
9
8
4
3
3
3
2
2
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
72
18
13
3
1
Host
Goat
35
19
18
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
66
41
21
19
10
6
5
2
2
1
1
Supplier
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Immunogen Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
Sequence C-RMKKLNSSDD GTQ
Specificity This antibody detects NRAS.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name NRAS (NRAS Antibody Abstract)
Background NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
Molecular Weight 21 kDa (NP_002515.1).
Gene ID 4893, 9606
UniProt P01111
Research Area Cancer
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-GTPase NRas antibody (NRAS) (Internal Region) (ABIN375000) anti-GTPase NRas (NRAS) (Internal Region) antibody
 image for anti-GTPase NRas antibody (NRAS) (Internal Region) (ABIN375000) anti-GTPase NRas (NRAS) (Internal Region) antibody (Image 2)
Background publications Thomas, Edmiston, Alexander et al.: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007 (PubMed).