GTPase NRas (NRAS) (Internal Region) antibody

Details for Product No. ABIN375000
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Antigen
Synonyms NRAS, AV095280, N-ras, ALPS4, NRAS1, NS6, N-RAS, fj99a10, wu:fj99a10, zgc:100761, zras-b1, H-RAS, HRAS
Epitope
Internal Region
(6), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(45), (20), (17), (12), (12), (3), (1)
Host
Goat
(32), (8), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(36), (18), (16), (10), (8), (4), (4), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN375000
357.50 $
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Immunogen Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
Sequence C-RMKKLNSSDD GTQ
Specificity This antibody detects NRAS. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat and Canine (Dog).
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name NRAS
Background NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Alternate names: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
Molecular Weight 21 kDa (NP_002515.1).
Gene ID 4893, 9606
UniProt P01111
Research Area Cancer
Application Notes Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 µg/ml. Approx.21kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21kDa and25kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 µg/ml. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-GTPase NRas (NRAS) (Internal Region) antibody anti-GTPase NRas (NRAS) (Internal Region) antibody
anti-GTPase NRas (NRAS) (Internal Region) antibody (2) anti-GTPase NRas (NRAS) (Internal Region) antibody (Image 2)
Background publications Thomas, Edmiston, Alexander et al.: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007 (PubMed).

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