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Details for Product No. ABIN375000

GTPase NRas (NRAS) (Internal Region) antibody

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Antigen
Synonyms ALPS4, N-ras, NRAS1, NS6, AV095280, fj99a10, zras-b1, wu:fj99a10, zgc:100761, N-RAS, NRAS
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN375000
Quantity 0.1 mg
Price
346.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
Sequence C-RMKKLNSSDDGTQ
Specificity This antibody detects NRAS. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat and Canine (Dog).
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name NRAS
Background NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Alternate names: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
Molecular Weight 21 kDa (NP_002515.1).
Gene ID 4893
NCBI Accession 9606
UniProt P01111
Research Area Cancer
Application Notes Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 µg/ml. Approx.21kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21kDa and25kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 µg/ml. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Thomas, Edmiston, Alexander et al.: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007 (PubMed).

Alternatives for antigen "GTPase NRas (NRAS)", type "Antibodies"
Hosts (33), (7), (6)
Reactivities (44), (20), (18), (13), (13), (3), (2), (1)
Applications (34), (19), (17), (10), (7), (5), (4), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (5), (5), (3), (3), (2), (2), (1)
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