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GTPase NRas antibody (Internal Region)

Verified NRAS Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN302138
  • Target See all GTPase NRas (NRAS) Antibodies
    GTPase NRas (NRAS)
    Binding Specificity
    • 11
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 45
    • 20
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 44
    • 12
    Goat
    Clonality
    • 41
    • 15
    Polyclonal
    Conjugate
    • 36
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GTPase NRas antibody is un-conjugated
    Application
    • 45
    • 17
    • 9
    • 7
    • 7
    • 7
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Purpose
    NRAS
    Sequence
    RMKKLNSSDD GTQ
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    GTPase NRas (NRAS)
    Alternative Name
    NRAS (NRAS Products)
    Synonyms
    NRAS antibody, AV095280 antibody, N-ras antibody, ALPS4 antibody, NRAS1 antibody, NS6 antibody, N-RAS antibody, fj99a10 antibody, wu:fj99a10 antibody, zgc:100761 antibody, zras-b1 antibody, H-RAS antibody, HRAS antibody, GTPase NRas antibody, neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog antibody, NRAS proto-oncogene, GTPase antibody, neuroblastoma ras oncogene antibody, neuroblastoma RAS viral oncogene homolog antibody, Tsp_01117 antibody, nras.L antibody, Nras antibody, NRAS antibody, nras antibody
    Background
    NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog
    Gene ID
    4893, 18176, 24605
    NCBI Accession
    NP_002515
    Pathways
    p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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