ERN1 antibody (Internal Region)

Catalog No. ABIN375027

Product Details anti-ERN1 Antibody

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to ERN1 / IRE1a.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine and Swine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ERN1 antibody (ABIN375027)

Lai, Li, Kuang, Cui, Yang, Sun, Liu, Chen, Yan, Wen: "PKCδ silencing alleviates saturated fatty acid induced ER stress by enhancing SERCA activity." in: Bioscience reports, Vol. 37, Issue 6, (2018) (PubMed).

Yokouchi, Hiramatsu, Hayakawa, Okamura, Du, Kasai, Takano, Shitamura, Shimada, Yao, Kitamura: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." in: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, (2008) (PubMed).

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1 / IRE1 (ERN1 Products)

Synonyms: IRE1 antibody, IRE1P antibody, IRE1a antibody, hIRE1p antibody, 9030414B18Rik antibody, AI225830 antibody, C85377 antibody, Ire1a antibody, Ire1alpha antibody, Ire1p antibody, RGD1559716 antibody, Ern1 antibody, Ire1 antibody, Ire1b antibody, sb:eu1005 antibody, zgc:109932 antibody, endoplasmic reticulum to nucleus signaling 1 antibody, endoplasmic reticulum to nucleus signaling 2 antibody, endoplasmic reticulum (ER) to nucleus signalling 1 antibody, serine/threonine-protein kinase/endoribonuclease IRE1 antibody, endoplasmic reticulum (ER) to nucleus signalling 2 antibody, ERN1 antibody, ERN2 antibody, Ern1 antibody, LOC524719 antibody, Ern2 antibody, ern1 antibody

Background: IRE1 is involved in myo inositol biosynthesis and is implicated as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus. It is a transmembrane protein that has both serine threonine kinase and endoribonuclease activities. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER.Synonyms: Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, IRE1p, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase/endoribonuclease IRE1

Gene ID: 2081

NCBI Accession: NP_001424

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response