Cytokeratin 13 antibody (C-Term, Isoform A)

Catalog No. ABIN375046

Product Details anti-Cytokeratin 13 Antibody

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Binding Specificity: C-Term, Isoform A

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to KRT13. This antibody is expected to recognize the reported isoform a only.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-TSNASGRRTSDVRRP, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/128000. Western blot: Approx.55 kDa band observed in Human Lung, Skeletal Muscle and Skinlysates (calculated MW of 49.5 kDa according to NP_705694.2). The observed molecularweight corresponds to earlier findings in literature with different antibodies (Sajjan,Microbiology.2003 Apr,149(Pt 4): 961-71. , PMID: 12686638). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Human Tonsil shows strong layered,filamentous staining. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cytokeratin 13

Target: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Alternative Name: Cytokeratin 13 (KRT13 Products)

Synonyms: krt13 antibody, CK13 antibody, K13 antibody, Ka13 antibody, Krt-1.13 antibody, Krt1-13 antibody, ck13 antibody, k13 antibody, keratin 24 antibody, keratin 13 antibody, keratin 13, type I S homeolog antibody, krt24 antibody, KRT13 antibody, Krt13 antibody, krt13.S antibody, k13 antibody

Background: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.Synonyms: CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13

Gene ID: 3860, 9606

UniProt: P13646