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GAD antibody (Glutamate Decarboxylase 1 (Brain, 67kDa)) (Internal Region)

Details for Product anti-GAD1 Antibody No. ABIN375070, Supplier: Log in to see
Antigen
  • GAD
  • cpsq1
  • gad1
  • gad1-A
  • gad67
  • GAD67
  • GAD2
  • AO090005000539
  • AO090103000342
  • EP10
  • GAD25
  • GAD44
  • Gad-1
  • Gad67
  • GAD1
  • GLUTAMATE DECARBOXYLASE
  • GLUTAMATE DECARBOXYLASE 1
  • MKP11.30
  • MKP11_30
  • glutamate decarboxylase
  • CPSQ1
  • SCP
  • GAD-67
  • AW822034
  • C88048
  • PGP 9.5
  • PGP9.5
  • R75593
  • UCH-L1
  • UCHL-1
  • gad
Epitope
Internal Region
36
19
16
15
13
8
6
6
6
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
142
67
59
9
6
6
2
1
1
1
1
1
Host
Goat
117
28
18
3
Clonality
Polyclonal
Conjugate
This GAD antibody is un-conjugated
7
7
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
131
66
38
30
11
10
7
7
4
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence from the internal region of the protein sequence
Sequence C-PDSPQRREKL HK
Specificity This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine, Bovine, Porcine.
Species reactivity (tested):Human.
Purification Affinity Chromatography
Alternative Name GAD1 / GAD67 (GAD1 Antibody Abstract)
Background Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain. There are two isoforms of GAD, GAD65 and GAD67 (65 and 67 kDa, respectively) that are encoded by two different genes. The two isoforms have distinct intraneuronal distribution in the brain. There is 64 % amino acid identity between the two isoforms, with the highest diversity located at the N terminus, which in GAD65 is required for targeting the enzyme to GABA containing secretory vesicles. GAD65 is primarily found in nerve terminals. GAD67 has been thought to be primarily located in the nerve cell body and has been identified as a major autoantigen in type 1 diabetes.Synonyms: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform
Molecular Weight 66.9kDa according to NP_000808.2
Gene ID 2571
NCBI Accession NP_000808
UniProt Q99259
Application Notes Peptide ELISA: 1/8000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-GAD antibody (Glutamate Decarboxylase 1 (Brain, 67kDa)) (Internal Region) (ABIN375070) anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibody
Background publications Huang, Akbarian: "GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia." in: PLoS ONE, Vol. 2, Issue 8, pp. e809, 2007 (PubMed).