Peptide ELISA: 1/8000. Western blot: Approx 16 kDa band observed in Human Peripheral Blood Mononucleocyteslysates (calculated MW of 13.6 kDa according to NP_002956.1). The observed molecularweight corresponds to earlier findings (approx. 16 kDa) in literature with different antibodies. Recommended concentration: 0.3 - 1 μg/mL. Immunohistochemistry on paraffin sections: Human Lung shows nuclear staining in selectcells. Recommended concentration: 2 - 4 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0,5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % sodium azide as preservative and 0.5 % bovine serum albumin as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.