MED15 antibody (both Isoforms, Internal Region)

Catalog No. ABIN375100

This Goat Polyclonal antibody specifically detects MED15 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for MED15 antibody (both Isoforms, Internal Region) (ABIN375100)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED15 Antibody

Binding Specificity: both Isoforms, Internal Region

Specificity: This antibody reacts to PCQAP / MED15.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-PPQSQTQPLVSQAQ, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Background: PCQAP / MED15 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.Synonyms: ARC105, Activator-recruited cofactor 105 kDa component, CTG repeat protein 7a, CTG7A, Mediator complex subunit 1, Mediator of RNA polymerase II transcription subunit 15, PCQAP, Positive cofactor 2 glutamine/Q-rich-associated protein, TIG1, TNRC7, TPA-inducible gene 1 protein, Trinucleotide repeat-containing gene 7 protein

Gene ID: 51586, 9606

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha