MED15 antibody (AA 450-500)

Catalog No. ABIN7453728

Product Details anti-MED15 Antibody

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Binding Specificity: AA 450-500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-MED15 Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 450 and 500

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15 (MED15 Products)

Synonyms: Arc105 antibody, BcDNA:GH03922 antibody, CG4184 antibody, Dmel\\CG4184 antibody, Med15 antibody, Med9/ARC105 antibody, dARC105 antibody, med15 antibody, PCQAP antibody, MED15 antibody, DKFZp459O0325 antibody, ARC105 antibody, CAG7A antibody, CTG7A antibody, TIG-1 antibody, TIG1 antibody, TNRC7 antibody, A230074L19Rik antibody, AW536074 antibody, Pcqap antibody, arc105 antibody, fi85b10 antibody, pcqap antibody, wu:fd19g01 antibody, wu:fi19d01 antibody, wu:fi85b10 antibody, zgc:55839 antibody, Mediator complex subunit 15 antibody, mediator complex subunit 15 antibody, mediator of RNA polymerase II transcription subunit 15 antibody, mediator complex subunit 15 L homeolog antibody, MED15 antibody, LOC100195848 antibody, Med15 antibody, med15 antibody, med15.L antibody

Background: Background: Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) Q96RN5].

Gene ID: 51586

NCBI Accession: NP_001003891

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha