MED15 antibody (C-Term)

Catalog No. ABIN6242465

This Rabbit Polyclonal antibody specifically detects MED15 in WB and IHC (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for MED15 antibody (C-Term) (ABIN6242465)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB51171

Product Details anti-MED15 Antibody

Binding Specificity: AA 712-746, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: Med15

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).

Molecular Weight: 86607

UniProt: Q924H2

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha