NDEL1 antibody (both Isoforms, Internal Region)

Catalog No. ABIN375101

Product Details anti-NDEL1 Antibody

Target: NDEL1 (NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1 (NDEL1))

Binding Specificity: both Isoforms, Internal Region

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NDEL1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to NDEL1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence CRNFAKDQASRKSY, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDEL1

Target: NDEL1 (NudE Nuclear Distribution E Homolog (A. Nidulans)-Like 1 (NDEL1))

Alternative Name: NDEL (NDEL1 Products)

Synonyms: EOPA antibody, MITAP1 antibody, NDE1L1 antibody, NDE2 antibody, NUDEL antibody, NUDE2 antibody, Nudel antibody, 2600006O07Rik antibody, mNudel antibody, wu:fi23c04 antibody, wu:fi23e06 antibody, eopa antibody, mitap1 antibody, ndel1 antibody, ndel1-a antibody, ndel1-b antibody, nudel antibody, nudE neurodevelopment protein 1 like 1 antibody, nudE neurodevelopment protein 1-like 1 antibody, nudE neurodevelopment protein 1-like 1b antibody, nudE neurodevelopment protein 1 like 1 L homeolog antibody, NDEL1 antibody, Ndel1 antibody, ndel1b antibody, ndel1.L antibody

Background: Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50 % identity with mouse and human NUDE proteins.Synonyms: EOPA, MITAP1, NUDEL, Nuclear distribution protein nudE-like 1

Gene ID: 81565, 9606

UniProt: Q9GZM8

Pathways: Regulation of Cell Size