ARNTL antibody (both Isoforms, Internal Region)

Catalog No. ABIN375102

Product Details anti-ARNTL Antibody

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Binding Specificity: both Isoforms, Internal Region

Reactivity: Human, Rat, Mouse, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ARNTL antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is expected to recognize both reported isoforms (NP_001025444.1, NP_001025443.1) of BMAL1

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine, Canine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence REKITTNCYKFKIKD, from the internal region of the protein sequence according to NP_001169.3, NP_001025444.1.

Application Details

Application Notes: Peptide ELISA: 1/8000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Alternative Name: ARNTL / BMAL1 (ARNTL Products)

Synonyms: BMAL1 antibody, BMAL1c antibody, JAP3 antibody, MOP3 antibody, PASD3 antibody, TIC antibody, bHLHe5 antibody, Bmal1 antibody, ARNTL antibody, xBmal1 antibody, arntl antibody, Arnt3 antibody, BMAL1b antibody, bmal1b' antibody, aryl hydrocarbon receptor nuclear translocator like antibody, aryl hydrocarbon receptor nuclear translocator-like antibody, aryl hydrocarbon receptor nuclear translocator like S homeolog antibody, ARNTL antibody, Arntl antibody, arntl.S antibody, arntl antibody

Background: BMAL1 is a putative transcription factor involved in the generation of biological rhythms. It activates cycling transcription of Period (PER) and Timeless (TIM) by binding to the E-box (3'-CACGTG-5') present in their promoters. BMAL1 gene cyc mutants don't display PER and TIM cycling, and are completely arrhythmic. Efficient DNA binding requires dimerization with another bHLH protein, BMAL1 forms a heterodimer with Clock in order to activate PER and TIM transcription.Synonyms: Aryl hydrocarbon receptor nuclear translocator-like protein 1, BHLHE5, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5, MOP3, Member of PAS protein 3, PAS domain-containing protein 3, PASD3, bHLH-PAS protein JAP3, bHLHe5

Gene ID: 406, 9606

UniProt: O00327

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effect