IDE antibody (Internal Region)

Catalog No. ABIN375106

This Goat Polyclonal antibody specifically detects IDE in WB. It exhibits reactivity toward Human and Dog.

Quick Overview for IDE antibody (Internal Region) (ABIN375106)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This IDE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IDE Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to Insulysin / Insulinase.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: Insulin-Degrading Enzyme

Background: Insulin degrading enzyme (IDE) has a preferential affinity for insulin such that the presence of insulin will inhibit IDE mediated degradation of other substrates. IDE degrades a variety of other peptides including atrial natriuretic peptide and amylin. IDE catabolizes A beta and has been implicated as a candidate enzyme responsible for the degradation and clearance of A beta in the brain. IDE has also been shown to degrade the APP intracellular domain (AICD), a product of gamma secretase cleaved APP that may function in nuclear signaling.Synonyms: Abeta-degrading protease, EC=3.4.24.56, IDE, Insulin protease, Insulinase, Insulysin

Gene ID: 3416, 9606

UniProt: P14735

Pathways: SARS-CoV-2 Protein Interactome