Peptide ELISA: Limit Dilution: 1/4000Western Blot: 1-3 μg/mL. Approx 30 kDa band observed in Human, Mouse and Rat SkeletalMuscle lysates (calculated MW of 31.0 kDa according to NP_004459.2). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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Background
FHL3 interacts with human phosphatase CDC25B in the cell nucleus. LIM domain proteins such as FHL3 are found to be important regulators in cell growth, cell fate determination, cell differentiation and remodeling of the cell cytoskeleton. Human FHL3 is a type of LIM only protein that contains four tandemly repeated LIM motifs with an N terminal single zinc finger (half LIM motif).Synonyms: FHL-3, Four and a half LIM domains protein 3, SLIM2, Skeletal muscle LIM-protein 2