DACH1 antibody (Internal Region)

Catalog No. ABIN375187

Product Details anti-DACH1 Antibody

Target: DACH1 (Dachshund 1 (DACH1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DACH1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to Dachshund homolog 1. This antibody is expected to recognise all three reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography

Immunogen: Peptide with sequence SPVENTPQNNECK, from the internal region of the protein sequence

Application Details

Application Notes: ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for DACH1

Target: DACH1 (Dachshund 1 (DACH1))

Alternative Name: DACH1 (DACH1 Products)

Synonyms: DACH antibody, AI182278 antibody, Dac antibody, Dach antibody, E130112M23Rik antibody, dach antibody, dachshund family transcription factor 1 antibody, dachshund 1 (Drosophila) antibody, dachshund family transcription factor 1 S homeolog antibody, dachshund homolog 1 antibody, DACH1 antibody, Dach1 antibody, dach1.S antibody, dach1 antibody, LOC100550020 antibody

Background: DACH1 is similar to the Drosophila melanogaster dachshund gene, which encodes a nuclear factor essential for determining cell fates in the eye, leg, and nervous system of the fly. It is a member of the Ski gene family and is thought to be involved in eye development. Four alternatively spliced transcripts encoding different isoforms have been described for this gene. (from Entrez gene). DACH1 has also been found to inhibit oncogene-mediated breast tumor growth via downregulation of cyclin D1. Expression of DACH1 has been found to inversely correlate with metastasis.Synonyms: (Drosophila), DACH, DACH-1, Dachshund homolog 1, FLJ10138, OTTHUMP00000040839, RP11-512J14.1

Gene ID: 1602

NCBI Accession: NP_004383

UniProt: Q9UI36

Pathways: Feeding Behaviour