EPH Receptor B2 antibody (Internal Region)

Catalog No. ABIN375195

The Goat Polyclonal anti-EPH Receptor B2 antibody (ABIN375195) specifically detects EPH Receptor B2 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for EPH Receptor B2 antibody (Internal Region) (ABIN375195)

Target: EPH Receptor B2 (EPHB2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EPH Receptor B2 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to EPHB2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: ammonium sulphate precipitation followed by antigen affinity chromatography

Immunogen: Peptide with sequence C-QKDRNHRPKFGQ, from the internal region of the protein sequence

Application Details

Application Notes: ELISA: 1/4000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: DRT, DRT, EPHT3, EPTH3, ERK, Ephrin type-B receptor 2, HEK5, NY-REN-47, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, TYRO5, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3

Gene ID: 2048

NCBI Accession: NP_004433

UniProt: P29323

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins