P2RX4 antibody (C-Term)

Catalog No. ABIN375223

Product Details anti-P2RX4 Antibody

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This P2RX4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-YREKKYKYVE DYEQ

Specificity: This antibody detects P2X4.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-P2RX4 antibody (ABIN375223)

Girón, Vílchez, Shreeram, Salto, Manzano, Cabrera, Campos, Edens, Rueda, López-Pedrosa: "β-Hydroxy-β-methylbutyrate (HMB) normalizes dexamethasone-induced autophagy-lysosomal pathway in skeletal muscle." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117520, (2016) (PubMed).

Millet, Martin, Bonnefoy, Saas, Mocek, Alkan, Terrier, Kerstein, Tamassia, Satyanarayanan, Ariel, Ribeil, Guillevin, Cassatella, Mueller, Thieblemont, Lamprecht, Mouthon, Perruche, Witko-Sarsat: "Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis." in: The Journal of clinical investigation, Vol. 125, Issue 11, pp. 4107-21, (2016) (PubMed).

Moshfegh, Aires, Kisielow, Vogel: "A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways." in: Scientific reports, Vol. 6, pp. 25104, (2016) (PubMed).

Nassar, Halle, Plagemann, Tzschentke et al.: "Detection of long-term influence of prenatal temperature stimulation on hypothalamic type-II iodothyronine deiodinase in juvenile female broiler chickens using a novel immunohistochemical ..." in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 179, pp. 120-4, (2015) (PubMed).

Ott, Plitzko, Krischkowski, Reichmann, Bittner, Mendel, Kunze, Clement, Havemeyer: "Reduction of sulfamethoxazole hydroxylamine (SMX-HA) by the mitochondrial amidoxime reducing component (mARC)." in: Chemical research in toxicology, Vol. 27, Issue 10, pp. 1687-95, (2015) (PubMed).

Grill, Neumann, Hiltwein, Kolligs, Schneider: "Intestinal E-cadherin Deficiency Aggravates Dextran Sodium Sulfate-Induced Colitis." in: Digestive diseases and sciences, Vol. 60, Issue 4, pp. 895-902, (2015) (PubMed).

Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

Cruz-Orengo, Daniels, Dorsey, Basak, Grajales-Reyes, McCandless, Piccio, Schmidt, Cross, Crosby, Klein: "Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility." in: The Journal of clinical investigation, Vol. 124, Issue 6, pp. 2571-84, (2014) (PubMed).

Morgan, Cheepala, Wang, Neale, Adachi, Nachagari, Leggas, Zhao, Boyd, Venkataramanan, Schuetz: "Deregulated hepatic metabolism exacerbates impaired testosterone production in Mrp4-deficient mice." in: The Journal of biological chemistry, Vol. 287, Issue 18, pp. 14456-66, (2012) (PubMed).

Freeman, Bowman, Zetter: "Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 3, pp. 907-15, (2011) (PubMed).

Chentoufi, Kritzer, Tran, Dasgupta, Lim, Yu, Afifi, Jiang, Carpenter, Osorio, Hsiang, Nesburn, Wechsler, BenMohamed: "The herpes simplex virus 1 latency-associated transcript promotes functional exhaustion of virus-specific CD8+ T cells in latently infected trigeminal ganglia: a novel immune evasion mechanism." in: Journal of virology, Vol. 85, Issue 17, pp. 9127-38, (2011) (PubMed).

Surgucheva, Shestopalov, Surguchov: "Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion cells." in: The Journal of biological chemistry, Vol. 283, Issue 52, pp. 36377-85, (2008) (PubMed).

Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, (2008) (PubMed).

Target Details for P2RX4

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Alternative Name: P2RX4 / P2X4 (P2RX4 Products)

Synonyms: P2X4 antibody, P2X4R antibody, AI504491 antibody, AW555605 antibody, D5Ertd444e antibody, p2rx4 antibody, p2x4 antibody, wu:fi03f02 antibody, p2x4r antibody, p2rx4.2 antibody, p2xr4.2 antibody, purinergic receptor P2X 4 antibody, purinergic receptor P2X, ligand-gated ion channel 4 antibody, purinergic receptor P2X, ligand-gated ion channel, 4a antibody, purinergic receptor P2X, ligand gated ion channel, 4 L homeolog antibody, purinergic receptor P2X, ligand-gated ion channel, 4b antibody, P2RX4 antibody, P2rx4 antibody, p2rx4a antibody, p2rx4.L antibody, p2rx4b antibody

Background: The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined. [provided by RefSeq]Synonyms: ATP receptor, P2X purinoceptor 4, Purinergic receptor

Molecular Weight: 43.4 kDa (NP_002551.2)

Gene ID: 5025

NCBI Accession: NP_002551

UniProt: Q99571

Pathways: Synaptic Membrane