P2RX4 antibody (C-Term)

Catalog No. ABIN334455

Cited in 2+ publications. The Goat Polyclonal anti-P2RX4 antibody (ABIN334455) specifically detects P2RX4 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for P2RX4 antibody (C-Term) (ABIN334455)

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This P2RX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-P2RX4 Antibody

Binding Specificity: C-Term

Purpose: P2X4 / P2X4R

Sequence: YREKKYKYVE DYEQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YREKKYKYVEDYEQ, from the C Terminus of the protein sequence according to NP_002551.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Kidney lysates and in preliminary testing of Human Liver lysate (calculated MW of 43.4 kDa according to NP_002551.2).This molecular weight is routinely observed by other sources and was successfully blocke

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-P2RX4 antibody (ABIN334455)

Freeman, Bowman, Zetter: "Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 3, pp. 907-15, (2011) (PubMed).

Yuan, Zhu, Zhou, Chen, Zhu, Ma, He, Tian, Shi: "Role of mast cell activation in inducing microglial cells to release neurotrophin." in: Journal of neuroscience research, Vol. 88, Issue 6, pp. 1348-54, (2010) (PubMed).

Target Details for P2RX4

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Alternative Name: P2RX4

Background: P2X4, P2X4R, P2RX4, purinergic receptor P2X, ligand-gated ion channel, 4, ATP receptor, ATP-gated cation channel protein, P2X purinoceptor 4, P2X receptor, subunit 4, purinergic receptor P2X ligand-gated ion channel 4, purinergic receptor P2X4, purinocept

Gene ID: 5025, 18438, 29659

NCBI Accession: NP_002551

Pathways: Synaptic Membrane