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Mouse anti-Rat IgG2a Antibody (Cy5)

ELISA, FACS Monoclonal 2A8F4 IgG1 Cy5
Catalog No. ABIN376056
  • Target See all IgG2a products
    IgG2a
    Reactivity
    • 98
    • 17
    • 1
    Rat
    Host
    • 57
    • 29
    • 13
    • 12
    • 5
    Mouse
    Clonality
    • 72
    • 25
    Monoclonal
    Conjugate
    • 22
    • 13
    • 13
    • 10
    • 9
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cy5
    Application
    • 57
    • 45
    • 38
    • 33
    • 24
    • 19
    • 11
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Flow Cytometry (FACS)
    Specificity
    Rat IgG2a
    Characteristics
    Mouse Anti-Rat IgG2a-CY5
    Purification
    Purified
    Immunogen
    Rat IgG2a hybridoma
    Clone
    2A8F4
    Isotype
    IgG1
  • Application Notes
    • Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , ELISPOT - Reported in literature
    • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, CY5, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:100 - 1:400 PE, CY5, and AF647 conjugates 1 g/mL
    Comment

    Excitation / Emission wavelength: 652 nm / 672 nm

    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    0.1 mg in 1.0 mL of PBS/Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Target
    IgG2a
    Abstract
    IgG2a Products
    Target Type
    Antibody
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