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Rat anti-Mouse lambda Antibody (PE)

FACS, ELISA Monoclonal JC5-1 IgG2b PE
Catalog No. ABIN376471
  • Target
    lambda
    Reactivity
    • 27
    • 13
    • 4
    Mouse
    Host
    • 26
    • 8
    • 7
    • 3
    Rat
    Clonality
    • 33
    • 11
    Monoclonal
    Conjugate
    • 8
    • 8
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    PE
    Application
    • 32
    • 31
    • 12
    • 12
    • 9
    • 9
    Flow Cytometry (FACS), ELISA
    Specificity
    Reacts with mouseκ 1 andκ 2 light chains. Does not react with other immunoglobulin isotypes or λ light chains.
    Characteristics
    Rat Anti-Mouse Lambda-PE
    Purification
    Purified
    Immunogen
    Unknown
    Clone
    JC5-1
    Isotype
    IgG2b
  • Application Notes
    • Applications: FC - Quality tested , ELISA - Quality tested FLISA - Quality tested IHC-FS - Reported in literature , WB-NR , Sep - Reported in literature
    • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate 1 g/mL
    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze!
    Protect conjugated products from light.

    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Peng, Eckhardt: "Role of the Igh intronic enhancer Eμ in clonal selection at the pre-B to immature B cell transition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 8, pp. 4399-411, (2013) (PubMed).

  • Target
    lambda
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