Goat anti-Mouse IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN376859

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA

Characteristics: Goat Anti-Mouse IgG(H+L), Rat ads-UNLB

Purification:

Purification Method: Affinity chromatography on mouse IgG covalently linked to agarose.

Preadsorption: Rat Adsorbed

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - ELISPOT , WB , IHC-FS , ICC
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, and AF555 conjugates 1:100 - 1:400 PE conjugate 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added. The fluorescein (FITC), rhodamine (TRITC), and Texas Red® (TXRD) conjugates are supplied as 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody