HA-Tag antibody

Details for Product No. ABIN387711, Supplier: Log in to see
Antigen
Reactivity
Tag
450
2
1
1
1
1
1
Host
Mouse
180
177
40
34
20
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
360
200
116
109
81
70
53
40
15
13
11
7
7
5
4
4
4
4
3
2
2
1
1
1
1
1
1
Options
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Brand THE™
Immunogen A KLH coupled synthetic peptide (YPYDVPDYA)
Sequence YPYDVPDYA
Clone 5E11D8
Isotype IgG1
Specificity THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
Characteristics Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
Purification Protein A affinity column
Alternative Name HA Tag
Target Type Tag
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Supplier Images
 image for anti-HA-Tag antibody (ABIN387711) Lane 1-3: GST fusion protein in E. coli lysate (~ 31 kD) Lane 4: Negative E. coli lys...
Product cited in: Cheng, Yu, Liu, Deng, Ma, Wang: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." in: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, 2015 (PubMed).

Tanner, Liu, Oberste, Pallansch, Collett, Kirkegaard: "Dominant drug targets suppress the emergence of antiviral resistance." in: eLife, Vol. 3, 2014 (PubMed).

Pillay, Selland, Fleisch, Leighton, Cheng, Famulski, Ritzel, March, Wang, Allison, Waskiewicz: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." in: Zebrafish, Vol. 10, Issue 3, pp. 353-64, 2013 (PubMed).

Shulga, Anderson, Topham, Epand: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, 2012 (PubMed).

Borroto-Escuela, Romero-Fernandez, García-Negredo, Correia, Garriga, Fuxe, Ciruela: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, 2011 (PubMed).