SUMO1 antibody

Catalog No. ABIN387785

Product Details anti-SUMO1 Antibody

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUMO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified recombinant GST-SUMO1 fusion protein was used as immunogen.

Clone: 66AT1273-94

Isotype: IgG1

Application Details

Application Notes: IF: 1:25. WB: 1:100~500. WB: 1:4000. IHC-P: 1:25. IHC-P: 1:25. FC: 1:25. IHC: 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SUMO1 antibody (ABIN387785)

Namanja, Li, Su, Wong, Lu, Colson, Wu, Li, Chen: "Insights into high affinity small ubiquitin-like modifier (SUMO) recognition by SUMO-interacting motifs (SIMs) revealed by a combination of NMR and peptide array analysis." in: The Journal of biological chemistry, Vol. 287, Issue 5, pp. 3231-40, (2012) (PubMed).

Truong, Lee, Chen: "Small ubiquitin-like modifier (SUMO) modification of E1 Cys domain inhibits E1 Cys domain enzymatic activity." in: The Journal of biological chemistry, Vol. 287, Issue 19, pp. 15154-63, (2012) (PubMed).

Li, Perkins, Su, Ma, Colson, Horne, Chen: "Gold nanoparticles as a platform for creating a multivalent poly-SUMO chain inhibitor that also augments ionizing radiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 11, pp. 4092-7, (2012) (PubMed).

Target Details for SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1 (SUMO1 Products)

Background: This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.

Molecular Weight: 11557

Gene ID: 7341

NCBI Accession: NP_001005781, NP_001005782, NP_003343

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway