Suppressor of Variegation 3-9 Homolog 2 (Drosophila) (SUV39H2) (N-Term) antibody

Antigen: Suppressor of Variegation 3-9 Homolog 2 (Drosophila) (SUV39H2)

Synonyms: KMT1B, AA536750, D2Ertd544e, 4930507K23Rik, D030054H19Rik, FLJ23414, SUV39H2, MGC128675

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN388081

Additional Information

Alternative name: SUV39H2

Gene ID: 79723

UniProt: NP_078946

Immunogen: This SUV39H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~70 amino acids from the N-terminal region of humanSUV39H2.

Isotype: Ig

Description: Other names: KMT1B

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SUV39H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~70 amino acids from the N-terminal region of humanSUV39H2.

Molecular Weight: 46682 DA

Comments:

Background: The murine gene Suv39h2 encodes an H3 histone methyltransferase (HMTase) 59% identical in sequence to mouse Suv39h1. During embryogenesis, both proteins overlap in tissue expression, yet Suv39h2 transcripts are restricted to the testes in adult animals. Immunolocalization of the Suv39h2 protein during spermatogenesis indicates enrichment at the heterochromatin from the leptotene to the round spermatid stage. Moreover, Suv39h2 specifically accumulates with chromatin of the sex chromosomes, which undergo transcriptional silencing during the first meiotic prophase. Suv39h2 HMTase may also organize meiotic heterochromatin with the potential for epigenetic imprint to the male germline.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

The anti-SUV39H2 Pab (ABIN388081) is used in Western blot to detect SUV39H2 in mouse kidney tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).